Arch Biochem Biophys. 2012 Nov 15;527(2):81-9.
Mediterranean diet polyphenols reduce inflammatory angiogenesis through MMP-9 and COX-2 inhibition in human vascular endothelial cells: a potentially protective mechanism in atherosclerotic vascular disease and cancer.
Scoditti E, Calabriso N, Massaro M, Pellegrino M, Storelli C, Martines G, De Caterina R, Carluccio MA.
Source
National Research Council (C.N.R.), Institute of Clinical Physiology, Lecce,
Italy.
Abstract
Diets with high content of antioxidant polyphenols are associated with low
prevalence of cardiovascular diseases and cancer. Inflammatory angiogenesis is a
key pathogenic process both in cancer and atherosclerosis, and is tightly
regulated by the proinflammatory enzyme cyclooxygenase (COX)-2 and the matrix
degrading enzymes matrix metalloproteinases (MMPs). We studied the effects of
antioxidant polyphenols from virgin olive oil (oleuropein and hydroxytyrosol)
and red wine (resveratrol and quercetin) on endothelial cell angiogenic response
in vitro, and explored underlying mechanisms. Cultured endothelial cells were
pre-incubated with 0.1-50 ?mol/L polyphenols before stimulation with phorbol
myristate acetate (PMA). All tested polyphenols reduced endothelial cell tube
formation on matrigel and migration in wound healing assays. The reduced
angiogenesis was associated with the inhibition of PMA-induced COX-2 protein
expression and prostanoid production, as well as MMP-9 protein release and
gelatinolytic activity. These effects were accompanied by a significant
reduction in the stimulated intracellular reactive oxygen species levels and in
the activation of the redox-sensitive transcription factor nuclear factor (NF)-?B.
Our findings reveal that olive oil and red wine polyphenols reduce inflammatory
angiogenesis in cultured endothelial cells, through MMP-9 and COX-2 inhibition,
supporting a potential protective role for dietary polyphenols in
atherosclerotic vascular disease and cancer.
Copyright © 2012 Elsevier Inc. All rights reserved.
PMID: 22595400 [PubMed - indexed for MEDLINE]